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Abstract:
Monobloc Le Fort III distraction osteogenesis allows superior skeletal advancement in treating severe syndromic craniosynostosis. We report a rare orbitalcomplication in a 3-year-old boy with Crouzon syndrome who developed right-eye exodeviation with limited abduction during the intradistraction period following this surgery. Images from a computed tomography scan confirmed direct impingement of the distracted right lateral orbital wall to the lateral rectus muscle. The impingement was surgically relieved via lateral orbital wall osteotomy. Ten months postdistraction, a review showed normal eye movement. Alateral orbital osteotomy cut for a monobloc Le Fort III distraction should be designed near the rim to prevent this complication. Source: CLEFT PALATE-CRANIOFACIAL JOURNAL Please cite as: Hariri F, Cheung LK, Rahman ZAA, Ramasamy SN, Ganesan D, Surg M. A rare orbital complication of eye exodeviation with limited abduction during monobloc Le Fort III distraction osteogenesis. Cleft Palate-Craniofacial Journal, 2015; 52(4):489-493 Abstract:
Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction. Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papayapulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) Results: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ Conclusion: In vitro upregulated synthesis of IL-6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoieticcytokines synthesis in cells of hematopoietic and mesenchymal origin. Source; BMC Complementary and Alternative Medicine Status: DOAJ as an open access journal at Biomedcentral Please cite as: Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MR. Carica papaya induces in vitro thrombopoietic cytokine secretion by mesenchymal stem cells and hemapoietic cells. BMC Complementary and Alternative Medicine, 2015; doi: 10.1186/s12906-015-0749-6 Abstract:
Objectives: The aim of this study was to conduct a comparative qualitative and quantitative assessment of the interfacial soft and hard tissues investingimplants and natural teeth. Materials and methods: The test sample consisted of six adult healthy male Macaca fascicularis with three-unit splinted crowns, each crown supported by an Ankylos screw-shaped titanium implant. These implants were placed in the mandibular premolar-second molar region, one side by an immediateloading (IL) and the other by delayed-loading (DL) protocol. The animals were sacrificed after 3 months of functional loading. Another two monkeys with natural dentition served as controls. Nondecalcified sections were prepared for assessment of optical intensities (OI) under a confocal laser scanning microscope. Results: In both the test (IL and DL) and control, the soft tissue complexes demonstrated a highly fluorescent keratinized layer and diminished cytoplasmic and enhanced membranous fluorescence in the remaining epithelium. Peri-implant mucosa was further characterized by an intense fluorescence at thejunctional epithelium-implant interface and in the stromal mononuclear infiltrate. Connective tissue contact and periodontal ligament were weakly fluorescent.In hard tissues, a high fluorescence was observed in peri-implant woven bone and along the implant-bone interface. Mean OI was significantly higher in peri-implant woven bone than around teeth (P<0.05). In the remaining soft and hard tissue complexes, no significant differences in mean OI between the test and control were observed (P>0.05). Conclusions: Present findings suggest that peri-implant woven bone is highly mineralized, while the peri-implant and gingival mucosa share structural similarities. Clinical relevance: Optical intensities of interfacial tissues investing implants and teeth are related to their biological properties. Source: CLINICAL ORAL INVESTIGATIONS Status: A paid open access option is available for this journal at Springer Please cite as: Siar CH, Toh CG, Romanos GE, Ng KH. Comparative assessment of the interfacial soft and hard tissues investing implants and natural teeth in the macaque mandible. Clinical Oral Invesigation, 2015; 19(6): 1353-1362 Abstract:
Breast cancer is one of the major issues in the field of oncology, reported with a higher prevalence rate in women worldwide. In attempt to reveal the potential biomarkers for breast cancer, the findings of differentially glycosylated haptoglobin and osteonectin in previous study have drawn our attention towardsglycoproteins of secretome from the MCF-7 cancer cell line. In the present study, further analyses were performed on the medium of MCF-7 cells by subjecting it to two-dimensional analyses followed by image analysis in contrast to the medium of human mammary epithelial cells (HMEpC) as a negative control. Carboxypeptidase A4 (CPA4), alpha-1-antitrypsin (AAT), haptoglobin (HP), and HSC70 were detected in the medium of MCF-7, while only CPA4 and osteonectin (ON) were detected in HMEpC medium. In addition, CPA4 was detected as upregulated in the MCF-7 medium. Further analysis by lectin showed that CPA4, AAT, HP, and HSC70 were secreted as N-glycan in the medium of MCF-7, with HP also showing differentially N-glycosylated isoforms. For theHMEpC, only CPA4 was detected as N-glycan. No O-glycan was detected in the medium of HMEpC but MCF-7 expressed O-glycosylated CPA4 and HSC70. All these revealed that glycoproteins could be used as glycan-based biomarkers for the prognosis of breast cancer. Source: Biomed Research Interntainal Status: DOAJ as an open access journal at Hindawi Please Cite As: Tan AA, Phang WM, Gopinath SCB, Hashim OH, Kiew LV, Chen Y. Revealing glycoproteins in the secretome of MCF-7 human breast cancer cells. Biomed Research International, 2015; doi: 10.1155/2015/453289 Abstract:
Keratinases are proteolytic enzymes predominantly active when keratin substrates are available that attack disulfide bridges in the keratin to convert them from complex to simplified forms. Keratinases are essential in preparation of animal nutrients, protein supplements, leather manufacture, textile processing, detergent formulation, feather meal processing for feed and fertilizer, the pharmaceutical and biomedical industries, and waste management. Accordingly, it is necessary to develop a method for continuous production of keratinase from reliable sources that can be easily managed. Microbial keratinase is less expensive than conventionally produced keratinase and can be obtained from fungi, bacteria, and actinomycetes. In this overview, the expansion ofinformation about microbial keratinases and important considerations in keratinase production are discussed. Source: Biomed Research International Status: DOAJ as an open access journal at Hindawi Please cite as: Gopinath SCB, Anbu P, Lakshmipriya T, Tang TH, Chen Y, Hashim I, Ruslinda AR, Arshad MKM. Biotechnological aspects and perspective of microbial keratinase production. Biomed Research International, 2015; Article no: 140726 Abstract:
Keratinases are proteolytic enzymes predominantly active when keratin substrates are available that attack disulfide bridges in the keratin to convert them from complex to simplified forms. Keratinases are essential in preparation of animal nutrients, protein supplements, leather manufacture, textile processing, detergent formulation, feather meal processing for feed and fertilizer, the pharmaceutical and biomedical industries, and waste management. Accordingly, it is necessary to develop a method for continuous production of keratinase from reliable sources that can be easily managed. Microbial keratinase is less expensive than conventionally produced keratinase and can be obtained from fungi, bacteria, and actinomycetes. In this overview, the expansion ofinformation about microbial keratinases and important considerations in keratinase production are discussed. Source: BIOMED RESEARCH INTERNATIONAL Status: Free full text available at Hindawi Please Cite as: Gopinath SCB, Anbu P, Lakshmipriya T, Tang TH, Chen Y, Hashim I, Ruslinda AR, Arshad MKM. Biotechnological aspects and perspective of microbial keratinase production. Biomed Research International, 2015; Article no: 140726 Abstract:
Purpose: The aim of this paper was to introduce a new mechanism of thermal sensitivity in nanocarriers that results in a relatively low drug release at physiological temperature and rapid release of the encapsulated drug at hyperthermia and thermal ablation temperature range (4060 degrees C). Materials and methods: The nanocarriers were synthesised by coating mesoporous silica nanoparticles with a thin layer of polyacrylamide. The low gelation temperature ofthe protective shell provides preferred routes for drug diffusion when the nanocarriers are heated within the hyperthermia temperature range. In order to determine the gelation point of polyacrylamide shell, differential scanning calorimetry was used. Various chemical, morphological, thermal, as well as drugloading capacities of these nanocarriers were characterised and their drug release behaviour was examined using magnetic resonance - guided focused ultrasound (MRgFUS). Results: Drug release measurements at different temperatures using doxorubicin showed 11.5 +/- 2.4% leakage in aqueous solution at 37 degrees C after 30 min, while this value was significantly increased to 67.6 +/- 2.5% at 60 degrees C. A 39.2 +/- 2.2% release of doxorubicin was also obtained due to the sonication of drug-loaded nanoparticles for 5 x 20 s using MRgFUS. Conclusion: The nanocarriers developed do not exhibit a sharp transition temperature. However, a relatively high loading efficiency as well as rapid drug release at thermal ablation temperature range makes these nanostructures promising candidates for application as adjuvants to various thermal modalities such as radiofrequency and high intensity focused ultrasound. Source: INTERNATIONAL JOURNAL OF HYPERTHERMIA Status: A paid open access option is available for this journal. at TandF online Please cite as: Dabbagh A, Abdullah BJJ, Abu Kasim NH, Abdullah H, Hamdi M. A new mechanism of thermal sensitivity for rapid drug release and low systemic toxicity in hyperthermia and thermal ablation temperature ranges. International Journal of Hyperthermia., 2015; 31(4): 375-385 |